Nairobi, Kenya (1999), David Long (GLP consultant) & Andreas Edelmann (F. 1999). Although the main physiological effect of GLP-1 seems to be gastric emptying, GLP-1 is also believed to have an effect on insulin secretion physiologically. The difference observed between the two studies could be caused by the use of in vitro islets in the former study and the use of the freshly isolated whole pancreatic organ in this study. Two glass coverslips (3 × 10 mm, thickness 0.12 - 0.17 mm; Matsunami Glass Inc., Osaka, Japan) were set on 24 well plates before seeding and culturing MIN6 cells in DMEM supplemented with 10% FBS in a humidified atmosphere with 5% CO2 at 37_C overnight. In conclusion, our data suggest that, in pancreatic tissue sections from mice, rats, and humans, GLP-1 receptor immunoreactivity is restricted to endocrine _ cells. 1997) observed immunoreactive GLP-1Rs on 20% of _ cells; however, we could not confirm this result using freshly fixed paraffin-embedded pancreatic tissue.

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